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Marine sponges, including the crumb of bread sponge, Hymeniacidon sinapium, display allorejection responses to contact with conspecifics in both experimental and natural settings. These responses have been used to infer immunocompetence in a variety of marine invertebrates. However, larvae and juveniles from several marine sponge species fuse and form chimeras. Some of these chimeras persist, whereas others eventually break down, revealing a period of allogeneic non-responsiveness that varies depending on the species. Alternatively, for H. sinapium, most pairs of sibling post-larvae and juveniles that settle in contact initiate immediate allorecognition and show the same morphological response progression as the adults. This indicates that allorecognition and response occurs during early metamorphosis. Results from H. sinapium and other sponge species, in addition to annotations of sponge genomes, suggest that allorecognition and immunocompetence in sponges are mediated by distinct systems and may become functional at different times during or after metamorphosis for different species. Consequently, allorecognition may not be a good proxy for the onset of immunocompetence. 

IntroductionThe California purple sea urchin,Strongylocentrotus purpuratus, relies solely on an innate immune system to combat the many pathogens in the marine environment. One aspect of their molecular defenses is theSpTransformer(SpTrf) gene family that is upregulated in response to immune challenge. The gene sequences are highly variable both within and among animals and likely encode thousands of SpTrf isoforms within the sea urchin population. The native SpTrf proteins bind foreign targets and augment phagocytosis of a marineVibrio. A recombinant (r)SpTrf-E1-Ec protein produced byE. colialso bindsVibriobut does not augment phagocytosis. MethodsTo address the question of whether other rSpTrf isoforms function as opsonins and augment phagocytosis, six rSpTrf proteins were expressed in insect cells. ResultsThe rSpTrf proteins are larger than expected, are glycosylated, and one dimerized irreversibly. Each rSpTrf protein cross-linked to inert magnetic beads (rSpTrf::beads) results in different levels of surface binding and phagocytosis by phagocytes. Initial analysis shows that significantly more rSpTrf::beads associate with cells compared to control BSA::beads. Binding specificity was verified by pre-incubating the rSpTrf::beads with antibodies, which reduces the association with phagocytes. The different rSpTrf::beads show significant differences for cell surface binding and phagocytosis by phagocytes. Furthermore, there are differences among the three distinct types of phagocytes that show specific vs. constitutive binding and phagocytosis. ConclusionThese findings illustrate the complexity and effectiveness of the sea urchin innate immune system driven by the natSpTrf proteins and the phagocyte cell populations that act to neutralize a wide range of foreign pathogens. 

The SpTransformer (SpTrf) gene family in the purple sea urchin, Strongylocentrotus purpuratus, encodes immune response proteins. The genes are clustered, surrounded by short tandem repeats, and some are present in genomic segmental duplications. The genes share regions of sequence and include repeats in the coding exon. This complex structure is consistent with putative local genomic instability. Instability of the SpTrf gene cluster was tested by 10 days of growth of Escherichia coli harboring bacterial artificial chromosome (BAC) clones of sea urchin genomic DNA with inserts containing SpTrf genes. After the growth period, the BAC DNA inserts were analyzed for size and SpTrf gene content. Clones with multiple SpTrf genes showed a variety of deletions, including loss of one, most, or all genes from the cluster. Alternatively, a BAC insert with a single SpTrf gene was stable. BAC insert instability is consistent with variations in the gene family composition among sea urchins, the types of SpTrf genes in the family, and a reduction in the gene copy number in single coelomocytes. Based on the sequence variability among SpTrf genes within and among sea urchins, local genomic instability of the family may be important for driving sequence diversity in this gene family that would be of benefit to sea urchins in their arms race with marine microbes. 

Abstract BackgroundSpotting disease infects a variety of sea urchin species across many different marine locations. The disease is characterized by discrete lesions on the body surface composed of discolored necrotic tissue that cause the loss of all surface appendages within the lesioned area. A similar, but separate disease of sea urchins called bald sea urchin disease (BSUD) has overlapping symptoms with spotting disease, resulting in confusions in distinguishing the two diseases. Previous studies have focus on identifying the underlying causative agent of spotting disease, which has resulted in the identification of a wide array of pathogenic bacteria that vary based on location and sea urchin species. Our aim was to investigate the spotting disease infection by characterizing the microbiomes of the animal surface and various tissues. ResultsWe collected samples of the global body surface, the lesion surface, lesioned and non-lesioned body wall, and coelomic fluid, in addition to samples from healthy sea urchins. 16S rRNA gene was amplified and sequenced from the genomic DNA. Results show that the lesions are composed mainly of Cyclobacteriaceae, Cryomorphaceae, and a few other taxa, and that the microbial composition of lesions is the same for all infected sea urchins. Spotting disease also alters the microbial composition of the non-lesioned body wall and coelomic fluid of infected sea urchins. In our closed aquarium systems, sea urchins contracted spotting disease and BSUD separately and therefore direct comparisons could be made between the microbiomes from diseased and healthy sea urchins. ConclusionResults show that spotting disease and BSUD are separate diseases with distinct symptoms and distinct microbial compositions. Graphical abstract 

The extremely large slip that occurred on the shallow portion of the Japan Trench subduction zone during the 2011 Mw 9.1 Tohoku-oki earthquake directly contributed to the devastating tsunami that inundated the Pacific coast of Japan. International Ocean Discovery Program (IODP) Expedition 405 (Tracking Tsunamigenic Slip Across the Japan Trench) aimed to investigate the conditions and processes that facilitated the extremely shallow slip on the subduction interface during the 2011 Tohoku-oki earthquake to improve understanding of the factors that allow slip to the trench on subduction zones. Expedition 405 implemented a combined logging, coring, and observatory operational plan at two sites: Site C0026, ~8 km seaward of the Japan Trench, to characterize the input sediments to the subduction zone and Site C0019, ~6 km landward of the trench, where the plate boundary fault zone is present at ~825 meters below seafloor (mbsf). At Site C0026, the input section was logged to ~430 mbsf with a logging-while-drilling (LWD) assembly that characterized the succession of sediments and rocks from the seafloor to the basaltic rocks of the oceanic crust. Cores recovered from four holes as deep as 290 mbsf contain a sequence of hemipelagic and pelagic sediments that will be input into the shallow subduction system and therefore control both the localization of the plate boundary fault zone and the slip behavior of the plate boundary. Site C0019 was previously drilled in 2012 during Integrated Ocean Drilling Program Expedition 343 (Japan Trench Fast Drilling Project [JFAST]), and revisiting this site allowed temporal variations in the frontal prism and plate boundary fault zone to be evaluated. The LWD data to ~980 mbsf characterized the frontal prism, plate boundary fault zone, and lower plate to the basaltic volcanic rocks. Cores were recovered from multiple holes that contain a variety of muds from the frontal prism and the plate boundary fault zone, as well as lower plate materials. Comparison with the sediments from Site C0026 provides a basis to interpret the tectonic and sedimentological processes operating in the dynamic environment of the frontal prism. Cores from the plate boundary fault zone provide a unique window into the structural complexity of an active plate boundary fault that is known to host large seismic slip. Two borehole observatories were installed at Site C0019 that contain temperature sensors deployed to take measurements over a period of years and reveal the hydrogeologic structure of the shallow subduction system. These hugely successful drilling operations, combined with postexpedition work to measure the mechanical, frictional, paleomagnetic, and hydrogeologic properties of the core samples and to constrain the history of past seismic slip at Site C0019, will provide an unprecedented opportunity to advance our understanding of shallow subduction systems. Outreach during the expedition leveraged and elevated the success of the operations by sharing the outcomes with a variety of domestic and international audiences, including scientists, students, educators, stakeholders, and the general public. Thanks to the efforts of a large group of onboard outreach officers and their onshore support, activities included ship-to-shore broadcast events; interviews with science party members and crew; the publication of videos, blogs, magazine articles, and social media posts; and development of formalized classroom lesson plans and materials.